Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Serelaxin promotes the preservation of endothelial cell properties in both in vivo and in vitro by regulating candidate genes. (A) Heat map and (B) scatter plot show the genes with altered mRNA expression level in only TGFβ1-treated (x-axis) or in both TGFβ1- and Serelaxin-treated (y-axis) HCAECs. STEAP1, NOTCH1, JAGGED1, RAC1, DSP, GSC, ERBB3, FZD7, GSK3B and TGFB2 were significantly regulated by Serelaxin treatment, which is shown by separation from dot lines (cut-off by 4 folds). (C-D) qPCR analysis showing the expression of candidate genes, which were identified from qPCR array in TGFβ1- and Serelaxin-treated MCECs. Cells without any treatment were used as control. (E) qPCR analysis of the panel of candidate genes in vivo in sham and AAC-operated mice treated with vehicle or Serelaxin. Serelaxin significantly rescued the effects of AAC on all genes except for Gsc and Steap1. Student t-test was used for single comparison and one-way ANOVA with Bonferroni post-hoc analysis was used for multiple group comparisons. Gene expression and associated error bars, representing mean ± SEM n≥3, n.s. no significance, * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Preserving, In Vivo, In Vitro, Expressing, Control, Comparison, Gene Expression

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Serelaxin rescues the Notch1 pathway in AAC-operated hearts. Immunofluorescence staining of (A) Jagged1, (B) Notch1 and (C) NICD in combination with WGA and DAPI in sham and AAC-operated hearts treated with vehicle or Serelaxin. Quantifications showed that protein expressions of (D) Jagged1, (E) Notch1 and (F) NICD were downregulated in AAC-operated hearts and could be restored by Serelaxin. (G) Western blot analysis shows the amount of the soluble form of Jagged1 in the medium of TGFβ1-treated MCECs. Ponceau-S stained membrane picture indicates that an equal amount of total precipitated protein was loaded for both the control and TGFβ1-treated MCECs. Soluble Jagged1 was reduced in TGFβ1-treated MCECs as quantified by densitometry analysis (right panel). Student t-test was used for single comparison and one-way ANOVA with Bonferroni post-hoc analysis was used for multiple group comparisons. Gene expression and associated error bars, representing mean ± SEM, n≥3, n.s. no significance, * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Immunofluorescence, Staining, Western Blot, Membrane, Control, Comparison, Gene Expression

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Antibodies used in Immunofluorescence staining

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Immunofluorescence

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Primers used in qPCR assay for gene expression analysis

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Gene Expression, Sequencing

Journal: Journal of Diabetes Research

Article Title: The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats

doi: 10.1155/2017/8968523

Figure Lengend Snippet: The mRNA expressions of Notch1 (a), RBP-Jk (b), Msx2 (c), and Jagged1 (d) in aortic tissues. Data presented as mean ± standard deviation (SD). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus Nor group; Δ p > 0.05, # p < 0.05, ## p < 0.01, and ### p < 0.001. Nor group: normal controls; DN + VDN group: diabetic nephropathy rats with vitamin D3/nicotine-induced vascular calcification.

Article Snippet: The following primary antibodies were used for immunohistochemical analysis: monoclonal rabbit anti-rat RBP-Jk, Msx2, and N1-ICD (Shanghai Bioworld Tech Inc., China); monoclonal rabbit anti-rat Jagged1 and Runx2 (Beijing BioTech Inc., China); monoclonal rabbit anti-rat Notch1 (Wuhan Proteintech Group Inc., China); and monoclonal mouse anti-rat α -SMA (Wuhan Boster Bio-Tech., Ltd, China).

Techniques: Standard Deviation

Journal: Journal of Diabetes Research

Article Title: The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats

doi: 10.1155/2017/8968523

Figure Lengend Snippet: Detection of Notch1, RBP-Jk, Msx2, Jagged1, and N1-ICD levels by immunohistochemical analysis. The representative images and quantitative analysis of Notch1 (a-b), RBP-Jk (c-d), Msx2 (e-f), Jagged1 (g-h), and N1-ICD (i-j) in aortic tissues. The left panel of histological images: Nor group; right panel: DN + VDN group at 8, 12 , and 16 weeks, respectively. Data presented as mean ± standard deviation (SD). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus Nor group; Δ p > 0.05, # p < 0.05, ## p < 0.01, and ### p < 0.001. Nor group: normal controls; DN + VDN group: diabetic nephropathy rats with vitamin D3/nicotine-induced vascular calcification.

Article Snippet: The following primary antibodies were used for immunohistochemical analysis: monoclonal rabbit anti-rat RBP-Jk, Msx2, and N1-ICD (Shanghai Bioworld Tech Inc., China); monoclonal rabbit anti-rat Jagged1 and Runx2 (Beijing BioTech Inc., China); monoclonal rabbit anti-rat Notch1 (Wuhan Proteintech Group Inc., China); and monoclonal mouse anti-rat α -SMA (Wuhan Boster Bio-Tech., Ltd, China).

Techniques: Immunohistochemical staining, Standard Deviation